Journal: Journal of functional foods

Article Title: Theaflavin-3, 3’-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway

doi: 10.1016/j.jff.2018.09.021

Figure Lengend Snippet: TF3 inhibited ovarian CSCs through Wnt/β-catenin signaling pathway. (A) Treatment with 20 μM TF3 downregulated the protein levels of β-Catenin in ALDH+ cells sorted from A2780/CP70 and OVCAR3 tumorspheres. (B) Treatment with 20 μM TF3 downregulated the protein levels of LEF-1, c-Myc and cyclin D1 in ALDH+ cells sorted from A2780/CP70 and OVCAR3 tumorspheres. (C) S33Y transfection upregulated the protein levels of β-Catenin in ALDH+ cells sorted from A2780/CP70 and OVCAR3 tumorspheres. (D) S33Y transfection attenuated the inhibitory effect of 20 μM TF3 on viability of the ALDH+ cell sorted from A2780/CP70 and OVCAR3 tumorspheres. (E) S33Y transfection attenuated the inhibitory effect of 20 μM TF3 on the sphere formation of ALDH+ cell sorted from A2780/CP70 and OVCAR3 tumorspheres. The tumorspheres were detected and photographed by Zeiss microscopy (magnification, ×40). Scale bar size is 100 μm. Data represent means ± SD of three independent experiments. * p< 0.05, ** p< 0.01, compared with respective controls.

Article Snippet: The cells were transfected with pcDNA3-S33Y β-Catenin plasmid (Addgene) using jetPrime transfection reagent (VWR International) following manufacturer’s protocol.

Techniques: Transfection, Microscopy

Journal: Scientific Reports

Article Title: Nrf2 depletion enhanced curcumin therapy effect in gastric cancer by inducing the excessive accumulation of ROS

doi: 10.1038/s41598-024-81375-1

Figure Lengend Snippet: Cur combined with Nrf2 knockdown effectively inhibited the proliferation, migration and invasion of GC cells. ( A , B ) rt-qPCR validated the efficiency of three siRNA sequences to knock down Nrf2 in AGS and HGC27 cells. ( C , D ) After GC cells were transfected with siNC and siNrf2 for 24 h, they were treated with Cur (0 and 20 µM) for 24 h. Cell viability was detected by the CCK8 assay. ( E – H ) The migratory capacity of GC cells was compared using a wound healing assay and the wound healing area was quantified. ( I – L ) GC cells were transfected with siNC and siNrf2 or treated with Cur (0 and 20 µM) for 24 h. Transwell assay was used to compare the migratory and invasive capacities of GC cells. Scale: 500 μm. Data are presented as the mean ± SD. n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: After incubating cells (2 × 10 5 /well) in 6-well plates for 24 h, jetPRIME in vitro siRNA transfection reagent (Yeasen, Shanghai, China) and complete media were added, along with small interfering RNAs (siRNAs) targeting Nrf2 (siNrf2) or negative control (NC) (50 nmol/l).

Techniques: Knockdown, Migration, Quantitative RT-PCR, Transfection, CCK-8 Assay, Wound Healing Assay, Transwell Assay